Table 1. Olympus Confocal Laser Scanning Microscopes
| Microscope | Illumination |
Excitation DM/Filter |
PMt1 | PMT2 | PMT3 |
Emission Filter |
|---|---|---|---|---|---|---|
|
FV1000 Main scanner |
405, 440, 488, 561, 633 nm |
BS 20/80 DM 405/488 DM 405/488/568/633 DM 458/515 DM 405-440/515 DM 405/488/543 |
Mirror Glass SDM560 SDM510 SDM490 |
Mirror Glass SDM640 SDM560 |
BA655-755 BA560-660 BA585-615 BA585-685 |
|
|
FV1000 SIM scanner |
405 nm |
ADM 504/473 ADM 405/488 ADM 450 ADM 515 ADM 559 ADM 720 ADM 650 |
||||
|
FV1000 Epi illumination |
Mercury 75W |
BP 400-440 BP 470-490 BP 460-490 (?) |
BA 475 BA 510 IF BA 510-550 |
|||
| DSU |
Xenon 75W (?) |
350/50 403/12 490/20 555/28 635/20 |
457/50 528/38 617/73 685/40 |
|||
|
Gemini FV1000 Main scanner |
405, 440, 488, 515, 559, 635 nm |
BS 20/80 DM 405/488 DM 405/488/559 DM 405/488/559/635 DM 458/515 DM 405-440/515 |
Mirror Glass SDM560 SDM510 SDM490 |
Mirror Glass SDM640 SDM560 |
BA655-755 BA575-675 BA575-620 BA465-495 |
|
|
Gemini FV1000 SIM scanner |
405, 440, 488, 515, 559, 635 nm |
ADM 405/473 ADM 405/488 ADM 450 ADM 515 ADM 559 ADM 720 RDM 650 |
||||
|
Gemini FV1000 Epi illumination |
Mercury 75W |
|||||
|
Gemini DSU |
Mercury 120W (X-Cite 120PC) |
CFP FRET mOrange mCherry |
CFP (480 nm) FRET (525 nm) mOrange (546 nm) mCherry (546 nm) |
|||
|
Injection Setup |
Xenon 75W (?) |
* DM = Dichroic mirror
* PMT = Photomultiplier tube
Table 2. Bio-Rad Confocal Laser Scanning Microscopes
| MICROSCOPE | ILLUMINATION |
EXCITATION DM/FILTER |
PMT1 | PMT2 | PMT3 |
EMISSION FILTER |
|---|---|---|---|---|---|---|
|
RTS 2000 Single photon |
440, 488, 568, 647 nm |
|
560LP (1:528/50, 2/3:open) 525LP (1:500/30) 100% 2/3 500LP(1:450/80, 2/3: open) |
Open(2:open, 3:525/50) 650LP (2:605/50, 3:700/75) 560LP(2:525/50, 3:605/70) DiA/I (2:550/30, 3:650/30) |
Open(2:open, 3:525/50) 650LP(2:605/50, 3:700/75) 560LP(2:525/50, 3:605/70) DiA/I (2:550/30, 3:650/30) |
|
|
RTS 2000 Two photon |
780~ 920 nm |
the same as above | the same as above | the same as above | ||
|
Radiance 2000 Single photon |
442, 488, 568, 637 nm |
100% Det1 440 DCLPXR 500 DCLPXR 560 DCLPXR 650 DCLPXR 100% Det 2/3 |
Blocking Filter: BGG22 E625SP HQ460/50 HQ475/40 ========= Emission Filter: HQ390/70 HQ450/80 D488/10 HQ500LP HQ515/30 HQ528/50 |
Blocking Filter: E525/70 HQ575/150 E625SP HQ540/50 HQ540/40 ======== Emission Filter: E460LP HQ515/30 HQ600/40 E570LP HQ590/70 HQ600/50 E600LP |
Blocking Filter: HQ575/150 HQ630/140M-2P ======= Emission Filter: E570LP HQ590/70 HQ600/50 E600LP HQ660LP |
|
|
Radiance 2000 Two photon |
850~ 920 nm |
None |
DM: 560DCLP 515DCLP ========== Filter: ET525/50M-2P CFP |
DM: None ========== Filter: ET620/60M-2P YFP |
||
| MRC 1024 |
488, 514, 568, 647 nm |
Triple DM 488/568/647 |
DM: 560DRLP ========== 605DF32 585EFLP 605DF32 |
522DF35 | 680DF32 | |
|
MRC 1024 Epi illumination |
Xenon 150W |
445 (GFP/YFP) DM510 (GFP, Nikon) DM580 (Nikon) CFP (Chroma) |
?? BA520 BA590 ?? |
Table 3. Nikon Microscopes
| MICROSCOPE | ILLUMINATION |
EXCITATION DM/FILTER |
PMT1 | PMT2 | PMT3 |
EMISSION FILTER |
|---|---|---|---|---|---|---|
|
Nikon RCM 8000 Single photon |
[400mW, KrAr,457~675nm] 488 514 nm (??) |
|||||
|
Nikon RCM 8000 Two photon |
Two-photon, 850-920 nm |
|||||
|
Nikon RCM 8000 Epi illumination |
Mercury 75W |
|||||
|
Nikon TE 2000 TIRF/PALM |
488, 568, 647 nm |
|||||
|
Nikon TE 2000 Yokogawa |
488, 568, 647 nm |
|||||
|
Nikon TE 2000 Epi illumination |
Mercury 75W |